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primescripttm ii 1st strand cdna synthesis kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm ii 1st strand cdna synthesis kit
    Primescripttm Ii 1st Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 11022 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+kit/PrimeScript+1st+strand+cDNA+Synthesis+Kit/pmc13123502-115-29-36
    Average 99 stars, based on 11022 article reviews
    primescripttm ii 1st strand cdna synthesis kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Synergistic effects of LCN2 and TWEAK on the progression of psoriasis
    Article Snippet: Total RNA was isolated from tissues or cells using TRIzol reagent (Invitrogen, Carlsbad, CA). .. Subsequently, the total RNA was reverse transcribed into cDNA using a cDNA kit (Takara Bio, Kyoto, Japan), followed by PCR amplification and quantification using SYBR Green Master Mixes (Takara Bio, Kyoto, Japan). .. The PCR primers were purchased from Tsingke Biotechnology (Beijing, China).

    Article Title: Synergistic effects of LCN2 and TWEAK on the progression of psoriasis.
    Article Snippet: Total RNA was isolated from tissues or cells using TRIzol reagent (Invitrogen, Carlsbad, CA). .. Subsequently, the total RNA was reverse transcribed into cDNA using a cDNA kit (Takara Bio, Kyoto, Japan), followed by PCR amplification and quantification using SYBR Green Master Mixes (Takara Bio, Kyoto, Japan). .. The PCR primers were purchased from Tsingke Biotechnology (Beijing, China).

    Article Title: LncRNA JINR1 regulates miR-216b-5p/ GRP78 and miR-1-3p/ DDX5 axis to promote JEV infection and cell death
    Article Snippet: .. PrimeScript first-strand cDNA kit ( # 6110A, Takara) was used for reverse transcription (RT) reaction with gene-specific primers (0.2 μM) for JEV/WNV 3′ UTR region. ..

    Article Title: Tissue Mimetic Membranes for Healing Augmentation of Tendon–Bone Interface in Rotator Cuff Repair
    Article Snippet: [ ] The extracted RNA was quantified using an ND‐2000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). .. Equal quantities (800 ng) of total RNAs from each sample were reverse transcribed to cDNA using a cDNA kit (Takara, cat# 634925). cDNA was amplified with TB Green qPCR SuperMix‐UDG (Takara) and specific primer sequences (Table , Supporting Information). .. The RT‐qPCRs were run on a QuantStudio 12K Flex Real‐time PCR system (Life Technologies, Thermo Fisher Scientific, USA).

    Polymerase Chain Reaction:

    Article Title: Synergistic effects of LCN2 and TWEAK on the progression of psoriasis
    Article Snippet: Total RNA was isolated from tissues or cells using TRIzol reagent (Invitrogen, Carlsbad, CA). .. Subsequently, the total RNA was reverse transcribed into cDNA using a cDNA kit (Takara Bio, Kyoto, Japan), followed by PCR amplification and quantification using SYBR Green Master Mixes (Takara Bio, Kyoto, Japan). .. The PCR primers were purchased from Tsingke Biotechnology (Beijing, China).

    Article Title: Synergistic effects of LCN2 and TWEAK on the progression of psoriasis.
    Article Snippet: Total RNA was isolated from tissues or cells using TRIzol reagent (Invitrogen, Carlsbad, CA). .. Subsequently, the total RNA was reverse transcribed into cDNA using a cDNA kit (Takara Bio, Kyoto, Japan), followed by PCR amplification and quantification using SYBR Green Master Mixes (Takara Bio, Kyoto, Japan). .. The PCR primers were purchased from Tsingke Biotechnology (Beijing, China).

    Amplification:

    Article Title: Synergistic effects of LCN2 and TWEAK on the progression of psoriasis
    Article Snippet: Total RNA was isolated from tissues or cells using TRIzol reagent (Invitrogen, Carlsbad, CA). .. Subsequently, the total RNA was reverse transcribed into cDNA using a cDNA kit (Takara Bio, Kyoto, Japan), followed by PCR amplification and quantification using SYBR Green Master Mixes (Takara Bio, Kyoto, Japan). .. The PCR primers were purchased from Tsingke Biotechnology (Beijing, China).

    Article Title: Synergistic effects of LCN2 and TWEAK on the progression of psoriasis.
    Article Snippet: Total RNA was isolated from tissues or cells using TRIzol reagent (Invitrogen, Carlsbad, CA). .. Subsequently, the total RNA was reverse transcribed into cDNA using a cDNA kit (Takara Bio, Kyoto, Japan), followed by PCR amplification and quantification using SYBR Green Master Mixes (Takara Bio, Kyoto, Japan). .. The PCR primers were purchased from Tsingke Biotechnology (Beijing, China).

    Article Title: Tissue Mimetic Membranes for Healing Augmentation of Tendon–Bone Interface in Rotator Cuff Repair
    Article Snippet: [ ] The extracted RNA was quantified using an ND‐2000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). .. Equal quantities (800 ng) of total RNAs from each sample were reverse transcribed to cDNA using a cDNA kit (Takara, cat# 634925). cDNA was amplified with TB Green qPCR SuperMix‐UDG (Takara) and specific primer sequences (Table , Supporting Information). .. The RT‐qPCRs were run on a QuantStudio 12K Flex Real‐time PCR system (Life Technologies, Thermo Fisher Scientific, USA).

    SYBR Green Assay:

    Article Title: Synergistic effects of LCN2 and TWEAK on the progression of psoriasis
    Article Snippet: Total RNA was isolated from tissues or cells using TRIzol reagent (Invitrogen, Carlsbad, CA). .. Subsequently, the total RNA was reverse transcribed into cDNA using a cDNA kit (Takara Bio, Kyoto, Japan), followed by PCR amplification and quantification using SYBR Green Master Mixes (Takara Bio, Kyoto, Japan). .. The PCR primers were purchased from Tsingke Biotechnology (Beijing, China).

    Article Title: Synergistic effects of LCN2 and TWEAK on the progression of psoriasis.
    Article Snippet: Total RNA was isolated from tissues or cells using TRIzol reagent (Invitrogen, Carlsbad, CA). .. Subsequently, the total RNA was reverse transcribed into cDNA using a cDNA kit (Takara Bio, Kyoto, Japan), followed by PCR amplification and quantification using SYBR Green Master Mixes (Takara Bio, Kyoto, Japan). .. The PCR primers were purchased from Tsingke Biotechnology (Beijing, China).

    Real-time Polymerase Chain Reaction:

    Article Title: Tissue Mimetic Membranes for Healing Augmentation of Tendon–Bone Interface in Rotator Cuff Repair
    Article Snippet: [ ] The extracted RNA was quantified using an ND‐2000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). .. Equal quantities (800 ng) of total RNAs from each sample were reverse transcribed to cDNA using a cDNA kit (Takara, cat# 634925). cDNA was amplified with TB Green qPCR SuperMix‐UDG (Takara) and specific primer sequences (Table , Supporting Information). .. The RT‐qPCRs were run on a QuantStudio 12K Flex Real‐time PCR system (Life Technologies, Thermo Fisher Scientific, USA).

    Synthesized:

    Article Title: hnRNPC Functions with HuR to Regulate Alternative Splicing in an m6A‐Dependent Manner and is Essential for Meiosis
    Article Snippet: The purity and integrity of the eluted RNA was assessed using an Agilent Bioanalyzer. .. 1–2 μg of cDNA was synthesized from the extracted RNA using the Clontech SMARTer cDNA KIT (Clontech Laboratories, CA, USA, Catalog# 634938) and adaptors were removed by digestion with RsaI. ..

    RNA Extraction:

    Article Title: GsMTx4-loaded GelMA promotes tendon regeneration and suppresses heterotopic ossification via the Apelin signaling pathway.
    Article Snippet: Tendon injuries pose significant challenges in both athletes and the general population, often leading to prolonged healing, impaired functionality, and increased risk of re-injury.. Current treatment options are limited and often yield unfavorable outcomes.. Given that tendons are highly mechanosensitive tissues, recent studies highlight the crucial role of mechanotransduction in tissue repair.

    Generated:

    Article Title: hnRNPC Functions with HuR to Regulate Alternative Splicing in an m6A‐Dependent Manner and is Essential for Meiosis
    Article Snippet: Total RNA was extracted from c‐KIT + cells of WT and Hnrnpc cKO mice at P8 using Direct‐zol RNA KIT (R2050, ZYMO Research, USA) according to the manufacturer's instructions. .. Strand‐specific libraries were generated using the Clontech SMARTer cDNA KIT (Clontech Laboratories, CA, USA, Catalog# 634938) and adaptors were removed by digestion with RsaI. ..



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